Charlie Tahan

Victor Frankenstein (voice)

Catherine O'Hara

Mrs. Frankenstein / Weird Girl / Gym Teacher (voice)

Martin Short

Mr. Frankenstein / Mr. Burgemeister / Nassor (voice)

Martin Landau

Mr. Rzykruski (voice)

Atticus Shaffer

Edgar 'E' Gore (voice)

Winona Ryder

Elsa Van Helsing (voice)

Robert Capron

Bob (voice)

James Hiroyuki Liao

Toshiaki (voice)

Conchata Ferrell

Bob's Mom (voice)

Tom Kenny

New Holland Towns Folk (voice)

Frank Welker

Sparky (voice) (uncredited)

Dee Bradley Baker

Persephone / Shelly / Were-Rat / Colossus / Mr. Whiskers / Driver (voice) (uncredited)

Jeff Bennett

Giant Sea Monkeys (voice) (uncredited)

Jon Donahue

Additional Voices (voice)